Over 30 years ago a new method was published that was referred to as 'western blotting' (Burnette 1981). In that article the author explained that the technique would be called 'western' blotting since a researcher named Southern had coined the analogous RNA blotting technique 'Southern blotting', and then a later DNA blotting technique had been similarly named 'northern blotting'. ("With due respect to Southern..., the established tradition of “geographic” naming of transfer techniques (“Southern,” “Northern”) is continued; the method described in this manuscript is referred to as “Western” blotting.")   In that article many of the initial conditions that are still in use were worked out, although it relied on radioactivity in a way that is no longer common. One of the figures is shown below, in which two different cell lines (in lanes A and B) were first subjected to SDS-PAGE and then suitable antibodies for detection of the specific protein: The article explains why there are so many bands detected, what the two cell lines were, and what the function of the protein is. It is mainly notable that since western blotting was not an established method yet (of course), it was not yet possible to purchase antibodies that were 'verified to work for western blotting' like we now often can. Therefore it was relatively difficult to choose a suitable protein for use in this article, and then difficult to obtain the suitable (antibody) reagents.   In the discussion section of this article, the author identified some possible challenges with this method. A notable point was "a disadvantage of the Western blot from SDS-gels is the tendency of antigenically reactive sites (epitopes) on the fractionated molecules to be irreversibly denatured by the detergent.... [With some antibodies] the reaction of interest may be abolished by the denaturing effect of the detergent."   Based on the information in this module, which one of the following best summarizes this concern?