If you were a genome researcher, exploring the genome of a newly discovered organism, and you were trying to determine if the organism’s genome contained any transposable elements, what characteristic features would you expect to see in the sequence at the site where a transposable element inserted a copy of itself into one of the organism’s chromosomes?

Question

BlackTom AI · Accepted Answer

When considering the genomic signature of a transposable element (TE) inserting into a chromosome, several patterns are commonly observed across many TE types.
Option 1: 'Flanking repeat' — This refers to target site duplications (TSDs) that arise from the insertion mechanism, creating short direct repeats on both sides of the inserted element. These are classic hallmarks of many DNA transposons and some retrotransposons, making flanking repeats a plausible feature at the insertion site.
Option 2: 'Inverted repeat' — Many DNA transposons have terminal inverted repeats (TIRs) at their ends, and after insertion those ends remain part of the inserted TE sequence, creating inverted-repeat boundaries flanking the internal transposon sequence. Observing inverted repeats adjacent to the inserted segment is another characteristic signal of transposable elements.
Option 3: 'A pigment gene' — While some TEs can carry accessory or regulatory genes in particular contexts, the presence of a pigment gene specifically at an insertion site is not a general or universal feature of TE insertions. Pigment genes are not a defining or reliably accompanying feature of TE insertions across organisms, so this is not a characteristic you would expect as a general rule.
Option 4: 'An enzyme coding gene (such as transposase)' — Some transposons encode their own transposase or related enzymes, but whether you would see such a gene at the insertion site depends on the TE family and the particular genomic context. The presence of a transposase gene is not a universal predicate of the site after insertion; many insertions may occur without bringing a functional transposase gene in the immediate site, especially if the TE has transferred previously-encoded machinery elsewhere in the genome. Therefore, this is not a guaranteed characteristic of the insertion site itself.
In summary, the most generally expected features at a TE insertion site are the presence of flanking repeats (target site duplications) and, for many DNA transposons, the inserted sequence is bordered by inverted repeats. The pigment gene and a standalone enzyme-coding gene at the exact insertion locus are not reliably expected features across TE insertions, making them less characteristic choices in this context.

01:447:380:17 GENETICS Week 5- In person problem set

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