Samples of three amino acids A, B and C, were spotted on the base line of a TLC plate. The chromatogram produced using a particular solvent is shown below:

Amino acids Rf values
alanine 0.56
isoleucine 0.79
taurine 0.34

Use the TLC plate and Rf values given to identify amino acid A.

Question

Samples of three amino acids A, B and C, were spotted on the base line of a TLC plate. The chromatogram produced using a particular solvent is shown below:

Amino acids	Rf values
			alanine	0.56
			isoleucine	0.79
			taurine	0.34

Use the TLC plate and Rf values given to identify amino acid A.

BlackTom AI · Accepted Answer

The question presents three amino acids (alanine, isoleucine, taurine) with their reported Rf values on a TLC plate: alanine 0.56, isoleucine 0.79, taurine 0.34. It asks to identify amino acid A using the TLC plate and these Rf values.

Because the options list is empty in the provided data, we cannot enumerate and assess multiple choices one by one. Instead, we proceed by matching the spot labeled A on the TLC plate to one of the known Rf values. In TLC, a higher Rf value indicates greater migration toward the solvent front; among the given values, 0.79 (isoleucine) is the most mobile, 0.56 corresponds to alanine, and 0.34 corresponds to taurine.

If the spot A shown on the chromatogram aligns with the position corresponding to the highest mobility among the listed spots, then A would be isoleucine (Rf ≈ 0.79). Conversely, if A aligned with the mid-position spot, it would be alanine (Rf ≈ 0.56), and if it aligned with the least-mobile spot, it would be taurine (Rf ≈ 0.34).

Key caveat: without viewing the actual TLC image, we cannot definitively assign A beyond the provided data; the conclusion depends on which spot A corresponds to on the chromatogram. The supplied final answer indicates isoleucine, which would be correct if A coincides with the spot at Rf ≈ 0.79.

To summarize the reasoning in a practical check:
- Compare the distance traveled by spot A to the solvent front with the reference Rf values: 0.34 (taurine), 0.56 (alanine), 0.79 (isoleucine).
- If A’s position matches the isoleucine spot (farther from origin, closest to solvent front), then A = isoleucine.
- If A’s position matches the alanine spot, then A = alanine; if it matches taurine, then A = taurine.

In this dataset, the designated answer is isoleucine, implying the A spot corresponds to the 0.79 Rf value on the provided TLC image.

4520_CHEM_X_0002 Computer Practical Exercises for Experiment 6F - Analysis of Spinach Extracts by TLC

Samples of three amino acids A, B and C, were spotted on the base line of a TLC plate. The chromatogram produced using a particular solvent is shown below: Amino acids Rf values alanine 0.56 isoleucine 0.79 taurine 0.34 Use the TLC plate and Rf values given to identify amino acid A.

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