True or False. When performing a dilution streak a new (or sterilized) loop is not required for each phase as long as the bacterial culture is pure.

Question

BlackTom AI · Accepted Answer

When evaluating a dilution streak protocol, the key concern is preventing cross-contamination between different sections of the plate and between sequential dilution steps.
Option 1: 'True' claims that a new or sterilized loop is not required for each phase as long as the bacterial culture is pure. In practice, even with a pure culture, using the same loop without sterilization between phases can introduce carryover of cells from one phase to the next, altering dilution accuracy and risking contamination of later stages. Therefore, this statement overlooks the standard aseptic practice of sterilizing between steps to maintain distinct dilution zones.
Option 2: 'False' asserts that you do need a new (or sterilized) loop for each phase to prevent cross-contamination and to preserve the integrity of the dilution series. This aligns with proper microbiological technique: each phase should be separated by sterilization or a fresh loop to ensure that cells from one dilution do not confound subsequent dilutions, even when the culture is pure. Neglecting this can lead to inaccurate counts and misleading results.
In summary, the reasoning behind the necessity of sterilizing or using a new loop between phases is about maintaining strict aseptic technique and accurate dilution, not about the purity of the culture alone. The original statement ignores this critical procedural safeguard, making it false.

True or False. When performing a dilution streak a new (or sterilized) loop is not required for each phase as long as the bacterial culture is pure.

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