The gel filtration chromatogram shown above is from a mixture of proteins. Choose below the appropriate sizes for the peaks shown. The molecular mass is in kD.

Question

BlackTom AI · Accepted Answer

To tackle this question, I start by identifying the general principle of gel filtration: larger proteins elute earlier (shorter elution time) than smaller proteins, so the first peak corresponds to the largest molecular mass, and subsequent peaks represent progressively smaller masses.
Option 1 lists the masses in ascending order (1: 15, 2: 25, 3: 30, 4: 50, 5: 70). Since peak 1 is the earliest/eluting peak, this would imply the smallest protein is eluting first, which contradicts the fundamental principle of size-exclusion chromatography. Therefore, option 1 is inconsistent with the expected trend.
Option 3 begins with 1: 70, 2: 30, 3: 30 plus a phosphate, 4: 25, 5: 15. While it places the largest mass first, it introduces an implausible entry for peak 3 ("30 plus a phosphate"), which is not a standard way to report a resolved protein size in gel filtration and hints at an extraneous or incorrect interpretation of a peak. This makes the sequence itself suspicious and biologically unclear.
Option 4 proposes 1: 70, 2: 30, 3: 50, 4: 75, 5: 15. Here the ordering is inconsistent with the elution principle as well: peak 4 is assigned 75 kD, larger than peak 1’s 70 kD, which would not be expected if peak 4 eluted after peak 1. Such a reversal undermines the basic logic of separation by size and suggests an incorrect set of assignments.
Option 2 states simply: "none of the answers make sense." Given the problems noted with options 1, 3, and 4—each containing either an incorrect elution ordering or biologically dubious annotations—it follows that none of the provided sequences reliably maps peaks to plausible molecular masses following the standard gel filtration behavior.
Overall, the common pitfall across the other options is either reversing the expected elution order or introducing nonstandard annotations (like a peak described as a mass plus a chemical modification in a way that isn’t typical for this plot). The safest conclusion from a conceptual standpoint is that the listed sequences do not correctly assign masses to peaks according to gel filtration principles, which aligns with selecting the option stating that none of the answers make sense.
Keep in mind, if you had the actual elution volumes or a calibration curve, you could map each peak to a specific kD more precisely, but with the information given and the standard interpretation, the other options fail to consistently reflect the expected trend.

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The gel filtration chromatogram shown above is from a mixture of proteins. Choose below the appropriate sizes for the peaks shown. The molecular mass is in kD.

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