You have treated a plasmid with different restriction enzymes. Given the plasmid map and gel below, answer the following questions: Which lane corresponds to treating the plasmid with EcoRI?

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Here is the step-by-step analysis of each answer option for the question about which lane corresponds to treating the plasmid with EcoRI.
Option a. I: Evaluate what EcoRI does to the plasmid map. If Lane I does not show the expected EcoRI restriction pattern (e.g., not the characteristic fragment sizes or banding) then this lane would be unlikely to represent EcoRI treatment. The mismatch implies I is not EcoRI.
Option b. II: The provided answer key states II as the EcoRI lane. If Lane II matches the distinctive EcoRI digestion pattern on the gel (the specific fragment sizes produced by a single EcoRI cut or the expected multi-fragment outcome for EcoRI on this plasmid), then II is the correct choice. This alignment with the map and enzyme site distribution supports II as EcoRI.
Option c. III: Consider whether Lane III corresponds to EcoRI. If Lane III shows a pattern inconsistent with EcoRI digestion (for example, a complete absence of change from the undigested control or a fragment pattern that matches another enzyme’s cuts), then III cannot be EcoRI.
Option d. IV: Similar reasoning applies to Lane IV. If the banding in IV does not fit the EcoRI digestion expectation (such as the wrong number or sizes of fragments compared to EcoRI’s cut map), this lane would be incorrect for EcoRI.
Option e. V: Finally, assess Lane V. If V’s pattern aligns with another enzyme’s specificity (or with undigested plasmid), not EcoRI, then V is not EcoRI.
Summarizing the reasoning, the EcoRI enzyme produces a specific set of fragment sizes based on its recognition sites on the plasmid map. The lane that best matches that predicted EcoRI pattern, given the map and gel, corresponds to II. Therefore, II is the correct lane for EcoRI treatment.

BPS3021 S1 2025 CYL | Cloning into a vector

You have treated a plasmid with different restriction enzymes. Given the plasmid map and gel below, answer the following questions: Which lane corresponds to treating the plasmid with EcoRI?

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