题目
SMC 2025 Spr - BIOL 3 (1286,1288) - Fundamentals of Biology (G) Quiz10 Biotechnology
单项选择题
Which of the following enzymes is an endonuclease?
选项
A.ligase
B.helicase
C.restriction enzyme
D.polymerase
查看解析
标准答案
Please login to view
思路分析
The question asks: Which of the following enzymes is an endonuclease?
Option 1: ligase. This enzyme seals nicks in the DNA backbone by forming a phosphodiester bond; it does not cut DNA, so it is n......Login to view full explanation登录即可查看完整答案
我们收录了全球超50000道考试原题与详细解析,现在登录,立即获得答案。
类似问题
You are trying to insert a human insulin gene into a bacterial plasmid. You cut the human DNA with the restriction enzyme EcoRI (which produces a specific sticky end) and you cut the plasmid with a different enzyme, BamHI (which produces a different sticky end). You then mix them with DNA ligase. What will be the result?
Question at position 5 In two separate reactions you digestion a large piece of DNA (700 kB). One you digest with RsaI (a 4-base cutter), the other you digest with BamHI (a 6-base cutter). You then run them on an agarose gel but forgot which lane was which. The sample digested with RsaI (assume complete digestion) is most likely to be _____________.The lane with the larger fragmentsThe lane with the smaller fragmentsThere is no way to tellThe lane that has visible fragments rather than a smearThe lane with the least number of fragments
Question28 How many times will the restriction enzyme DdeI, which has a recognition site of 5'- C/TNAG - 3', be expected to cleave a plasmid that is 5,822 base pairs in length? Round your answer to the nearest whole number and type it in the box provided below. Do not include punctuation in your answer. For example, if your answer is 1,000 type in 1000. DdeI will cleave the plasmid [input] time(s). Maximum marks: 1 Flag question undefined
Which condition would lead to a likely increase in the number of fragments produced during a restriction endonuclease (RE) digestion reaction?
更多留学生实用工具
希望你的学习变得更简单
加入我们,立即解锁 海量真题 与 独家解析,让复习快人一步!