The most useful restriction enzymes for creating recombinant DNA

Question

BlackTom AI · Accepted Answer

Interpreting the question, we are asked to identify which feature of restriction enzymes is most useful for creating recombinant DNA. 
Option 1: 'leave a sticky residue on the cut ends of the DNA.' This describes sticky ends, which result from staggered cuts and are indeed highly useful for ligating DNA fragments with complementary overhangs. However, the statement is somewhat awkward in wording—while it hints at sticky ends, it doesn’t explicitly tie this feature to the practical use in recombinant DNA construction. 
Option 2: 'also have the ability to ligate the ends of DNA back together.' Restriction enzymes do not ligate DNA; ligation is performed by DNA ligase. Therefore, this claim is inaccurate and misleading about the enzyme’s function. 
Option 3: 'cut DNA in a staggard pattern.' This option describes staggered (or "sticky") cuts that produce overhangs. These overhangs allow fragments with complementary sequences to anneal, making it much easier to join them in a controlled way, which is central to recombinant DNA techniques. The term 'staggard' is a misspelling of 'staggered,' but the concept conveyed aligns with sticky ends.
Option 4: 'cut the DNA bluntly at the ends.' Blunt cuts produce blunt ends, which lack overhangs and are harder to join in a specific orientation without additional processing. While blunt-ended fragments can be ligated, the efficiency and directionality are much lower, making blunt cutters less advantageous for purposeful recombinant constructs compared to sticky ends.
In evaluating the options, the distinguishing feature is whether the enzyme creates overhangs (sticky ends) or blunt ends, and whether it participates in ligation. The most advantageous property for constructing recombinant DNA is the creation of cohesive, complementary overhangs that facilitate precise ligation, which corresponds to the staggered-cut description. The other choices either misstate the enzyme’s capabilities (ligating DNA) or describe a less favorable end type (blunt ends) for recombination.

APBIO142-030 with labs Unit 6 Test

The most useful restriction enzymes for creating recombinant DNA

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