In SDS-PAGE, which protein migrate most quickly through the gel? Ones that are ______.

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In SDS-PAGE, proteins are denatured and coated with the detergent SDS, which imparts a uniform negative charge proportional to their length. This means migration through the polyacrylamide gel primarily reflects size (length of the polypeptide chain) rather than intrinsic charge or hydrophobicity.
Option 1: 'smallest' — Correct reasoning: smaller proteins have less mass and can traverse the gel matrix more quickly when carrying a similar charge-to-mass ratio, so they migrate the fastest.
Option 2: 'have a lot of highly negative R-groups' — In SDS-PAGE, the negative charges from SDS overwhelm the native charges of the amino acid side chains, so the presence of more negative R-groups does not drive faster migration.
Option 3: 'have a lot of highly positive R-groups' — Similarly, positive residues’ charges are neutralized by SDS, so their abundance does not cause faster movement through the gel.
Option 4: 'have a lot of hydrophobic R-groups' — Hydrophobicity is also largely masked by SDS coating and the denaturing conditions; migration is not governed by hydrophobic content in this assay.
In summary, the key determinant under SDS-PAGE conditions is the protein’s size after denaturation and SDS binding, making the smallest proteins migrate the fastest.

In SDS-PAGE, which protein migrate most quickly through the gel? Ones that are ______.

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In SDS-PAGE, which protein migrate most quickly through the gel? Ones that are ______.

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类似问题

When current is applied:

SDS-PAGE gels are made of acrylamide and bis-acrylamide since these two create a small sieving. Mark theINCORRECT statement

the SDS-PAGE gel shown above is the result of an experiment in which a ~55kDa protein is purified from cells, shown in Lane 3. What would you predict lanes 4, 5, and 6 show about the ~55kDa protein? Choose the correct answer from the answers below

SDS-PAGE is ____________ and separates molecules based on ____________

What is the purpose of SDS in SDS-PAGE? Breaks disulfide linkages Unfolds proteins to secondary structure Coats proteins in a negative charge

SDS-PAGE separates proteins mainly on the basis of __________?

In a consumer society, many adults channel creativity into buying things

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希望你的学习变得更简单

SDS-PAGE is and separates molecules based on