Which statement is true about the peptide bond in proteins?

Question

BlackTom AI · Accepted Answer

Restating the question: Which statement is true about the peptide bond in proteins?

Option a: It is freely rotating due to side-chain flexibility.
- This is incorrect. The peptide bond has partial double-bond character due to resonance between the carbonyl oxygen and the amide nitrogen, which restricts rotation about the C–N bond. Side-chain flexibility does not make the peptide bond freely rotatable.

Option b: It has a tetrahedral geometry due to sp³ hybridization.
- This is false. The peptide bond carbonyl carbon and the amide nitrogen adopt a planar arrangement (roughly sp² hybridization on those centers), not a tetrahedral geometry. The resonance gives rise to planarity of the amide bond.

Option c: It is planar and exhibits partial double-bond character.
- This is true. The peptide bond is planar because of resonance between the carbonyl and the amide nitrogen, which gives it partial double-bond character and restricts rotation, resulting in a flat configuration.

Option d: It forms only between charged amino acids.
- This is incorrect. Peptide bonds form between any amino acids, linking the carboxyl terminus of one amino acid to the amino terminus of the next, regardless of side-chain charge. Charge has nothing to do with the formation of the backbone bond.

Option e: It rotates around the omega angle to allow folding.
- This is misleading. While the backbone does have an omega angle, rotation about the peptide bond itself is largely restricted due to the partial double-bond character; the typical trans peptide bond fixes the geometry and does not freely rotate to facilitate folding. Therefore this statement overstates the ease of rotation and is not accurate as a general truth about peptide bonds.

In summary, each incorrect option misstates fundamental aspects of peptide bond structure or dynamics, while option c correctly captures the essential planarity and partial double-bond character that define the peptide bond.

BINF3010/BINF9010-Applied Bioinformatics - 2025 T2

Which statement is true about the peptide bond in proteins?

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