This is an H&E stained dog intestine (jejunum, 1250x). Which of the following statements is correct?

Question

BlackTom AI · Accepted Answer

First, let me restate the question and the available choices to set the context for evaluation.
Question: This is an H&E stained dog intestine (jejunum, 1250x). Which of the following statements is correct?
Options:
1) You can see a row of epithelial cells. The nuclei are stained in dark blue.
2) You can see part of an epithelial cell. The cell membrane is visible in the top and cellular structures such as lysosomes, peroxisomes and vesicles are clearly visible.

Now, evaluating each option in turn:
Option 1: This statement aligns with standard histology of H&E-stained intestinal tissue. Hematoxylin stains nuclei a dark blue to purple color, highlighting densely packed chromatin, while eosin stains the cytoplasm and extracellular matrix pink. In a jejunal cross-section at high magnification, you typically observe a row or lining of columnar epithelial cells with nuclei located basally or at varying heights along the crypt-villus axis. The emphasis on the nuclei being dark blue is consistent with H&E staining and the histological appearance of intestinal epithelium. This option is plausible and accurately describes recognizable features in an H&E slide of intestine.
Option 2: The claim that you can see part of an epithelial cell with the cell membrane clearly visible at the top, and that organelles such as lysosomes, peroxisomes, and vesicles are clearly visible, is not consistent with what H&E staining reveals at light microscopy at standard histological practice. H&E does not produce sharply defined membranes or discrete, easily identifiable intracellular organelles like lysosomes or peroxisomes in routine sections; those structures are more typically resolved with special stains or electron microscopy. Therefore, this option is anatomically and technically unlikely for an H&E-stained light microscope image of intestinal tissue at 1250x.
From a reasoning standpoint, the key distinctions are: (a) H&E highlights nuclei in blue/purple and cytoplasmic eosin staining; (b) intestinal epithelium presents as a row of columnar cells with nuclei visible, matching option 1; (c) explicit recognition of cell membranes and specific organelles as clearly visible is not characteristic of standard H&E images at this magnification, making option 2 inaccurate.
In summary, option 1 describes a correct, typical observation for an H&E stained jejunal section, while option 2 describes features that would not be expected or discernible in this context, pointing to a misinterpretation of what H&E can reveal at this scale.

AVBS2005 (ND) AEH P3 Quiz

This is an H&E stained dog intestine (jejunum, 1250x). Which of the following statements is correct?

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