What two 3-carbon molecules are generated by the cleavage of fructose-1,6-bisphosphate?

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BlackTom AI · Accepted Answer

Question: What two 3-carbon molecules are generated by the cleavage of fructose-1,6-bisphosphate?

Option A: glyceraldehyde-3-phosphate and 3-phosphoglycerate
This is not correct. Fructose-1,6-bisphosphate is cleaved into two 3-carbon triose phosphates, but the products are glyceraldehyde-3-phosphate (G3P) and dihydroxyacetone phosphate (DHAP), not 3-phosphoglycerate. 3-phosphoglycerate is a downstream glycolytic intermediate formed later, after the isomerization of DHAP to G3P.

Option B: glyceraldehyde-3-phosphate and dihydroxyacetone phosphate
This option accurately reflects the immediate products of the aldolase-catalyzed split of fructose-1,6-bisphosphate in glycolysis. The enzyme splits the six-carbon sugar into two three-carbon molecules: one becomes G3P, the other DHAP, which is rapidly isomerized to another molecule of G3P.

Option C: pyruvate and phosphoenolpyruvate
This pair are not direct products of this cleavage step. Pyruvate and phosphoenolpyruvate are downstream glycolytic/metabolic intermediates formed much later in glycolysis and gluconeogenesis, not from the initial split of fructose-1,6-bisphosphate.

Option D: enolase and 2-phosphoglycerate
Enolase is an enzyme involved in glycolysis, not a product. 2-phosphoglycerate is indeed an intermediate later in glycolysis, but it is produced after the conversion of 3-phosphoglycerate and 2-phosphoglycerate steps, not from the cleavage of fructose-1,6-bisphosphate.

Option E: glyceraldehyde-3-phosphate and pyruvate
This pairing includes G3P but pairs it with pyruvate, which is far downstream of the cleavage event. Pyruvate is formed after multiple subsequent steps of glycolysis under aerobic or anaerobic conditions, not directly from fructose-1,6-bisphosphate cleavage.

What two 3-carbon molecules are generated by the cleavage of fructose-1,6-bisphosphate?

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