Use the following description and the figure to determine if clone-by-clone sequencing or shotgun sequencing is being used.

The entire genome of Haemophilus influenzae – a bacterium that can cause ear infections and bloodstream infections – was broken down into small fragments. These fragments of the genome were then sequenced. After sequencing, software analysis was used to assemble the fragments by analyzing overlapping sequences.

Question

Use the following description and the figure to determine if clone-by-clone sequencing or shotgun sequencing is being used.

The entire genome of Haemophilus influenzae – a bacterium that can cause ear infections and bloodstream infections – was broken down into small fragments. These fragments of the genome were then sequenced. After sequencing, software analysis was used to assemble the fragments by analyzing overlapping sequences.

BlackTom AI · Accepted Answer

The question asks you to determine which sequencing approach is described by the given description and figure.
First, consider the process outlined: the entire genome is broken into small fragments, these fragments are sequenced, and then software is used to assemble the fragments by analyzing overlapping sequences. This sequence of steps is the hallmark of shotgun sequencing, where random fragments are generated and then computationally reassembled based on overlaps.
If we compare to the clone-by-clone (or map-based) method, the typical workflow involves first isolating large DNA clones (like BACs or cosmid clones) that cover the genome, then breaking those large clones into smaller pieces for sequencing, and finally assembling the genome by aligning overlapping regions from these large, predefined clones. This method relies on a hierarchical approach using known clone positions as anchors. In the description given, there is no mention of starting with large cloned fragments or using a hierarchical map-based assembly; instead, it emphasizes breaking the genome into small fragments first and assembling solely from overlaps, which aligns with shotgun sequencing.
Therefore, the described approach corresponds to shotgun sequencing rather than clone-by-clone sequencing. 
Note: If an option about clone-by-clone sequencing were present, you would expect emphasis on large clones and a hierarchical assembly strategy anchored by those clones, which is not described here. In contrast, the current description emphasizes fragment-based sequencing and overlap-based assembly without a hierarchical cloning framework, reinforcing shotgun sequencing as the correct interpretation.

BIOL_1105_60452_202506 Lesson 19 Homework

查看解析

登录即可查看完整答案

类似问题

Which of the following is least likely to be involved in whole-genome sequencing?

Another scientific race was described in the ‘Science’ paper on your LMS site. What race was this?

Public versus private funding was one of the big differences between the teams trying to sequence the human genome

Place the steps of sequencing a genome in order. 1: Annotating 2: Sequencing 3: Assembling

Place the steps of sequencing a genome in order. 1: Annotating 2: Sequencing 3: Assembling

True or False C. elegans was the first animal to have its genome fully sequenced.

更多留学生实用工具

考试浏览器助手

风格化写作助手

论文查重助手

文献引用助手

课堂转译助手

课堂笔记助手

Quiz搜索助手

学校历年真题

智能刷题助手

智能匹配练习

希望你的学习变得更简单