Which of the following represents the correct sequence of steps in assembling and annotating a plant genome?

Question

BlackTom AI · Accepted Answer

Considering the typical workflow for assembling and annotating a plant genome, we need to lay out the logical order of steps from raw material to annotated genome.
Option 1: 'Sequence the fragments → Fragment the genome → Stitch the sequences together → Labelling genomic regions' places sequencing before fragmentation, which is illogical because you cannot sequence something that hasn't been broken into manageable fragments yet.
Option 2: 'Fragment the genome → Sequence the fragments → Stitch the sequences together → Labelling genomic regions' presents a coherent pipeline: first you break the genome into pieces, then you sequence those pieces, next you assemble or stitch the reads into longer contiguous sequences, and finally you annotate or label genomic regions to interpret the structure.
Option 3: 'Stitch the sequences together → Fragment the genome → Sequence the fragments → Labelling genomic regions' starts with stitching before you have sequences or fragments; chaining from nothing to assembly is conceptually incorrect, making this order invalid.
Option 4: 'Fragment the genome → Labelling genomic regions → Sequence the fragments → Stitch the sequences together' inserts labeling before you have a complete assembly, which is premature and suggests annotation occurs prior to obtaining assembled contigs, violating the standard sequence of steps.
In summary, only Option 2 follows the logical, sequential progression from fragmentation to sequencing to assembly to annotation, whereas the other options disrupt the natural order at one or more points and would not produce a coherent genome assembly workflow.

AGRI30048_2025_SM2 Sample Final Exam Style Questions - unmarked

Which of the following represents the correct sequence of steps in assembling and annotating a plant genome?

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