In the field of genomics, what is “Genome Assembly”?

Question

BlackTom AI · Accepted Answer

Topic focus: Genome Assembly in genomics. 
Option 1: 'The process of creating multiple copies of a genome' — This is inaccurate. Creating multiple copies of a genome is more aligned with amplification or cloning processes, not assembly. Genome assembly is not about duplicating genomes, it’s about assembling pieces.
Option 2: 'The process of aligning and merging overlapping DNA fragments that are part of a genome' — This correctly captures the core idea of genome assembly. Sequencing generates many short fragments; assembly algorithms align overlapping regions to reconstruct the original longer genome sequence, stitching together contigs and scaffolds.
Option 3: 'The process of predicting the biological function of genes within a genome' — This describes functional annotation or gene prediction, not assembly. It happens after or alongside assembly but is a different step aimed at interpreting sequence data, not assembling it.
Option 4: 'The laboratory method where an organism's genome is prepared to be read by a sequencing instrument' — This sounds like library preparation or DNA preparation for sequencing, which is a preparatory step before sequencing, not the assembly of the genome itself.
In summary, option 2 directly describes the assembly task of combining overlapping fragments to reconstruct the genome, while the other options refer to amplification, annotation, or preparatory sequencing steps that are distinct from the assembly process.

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In the field of genomics, what is “Genome Assembly”?

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