True or False? Ultraviolet wavelengths of light are required to visualise plasma membranes of cells. (1 mark)

Question

BlackTom AI · Accepted Answer

The statement asks whether ultraviolet wavelengths are required to visualise plasma membranes of cells. 
Option 1: True. This is not correct because visualising plasma membranes does not necessitate UV light. Many common approaches use visible light with specific dyes or fluorescent tags that are excited in the blue, green, or red parts of the spectrum. UV is not a mandatory requirement for membrane imaging. 
Option 2: False. This is the correct choice. Plasma membranes can be visualised using a variety of methods that rely on visible light, such as membrane-specific dyes (e.g., lipophilic dyes that incorporate into the lipid bilayer) or fluorescent fusion proteins targeted to the membrane, as well as non-fluorescent techniques like phase-contrast or differential interference contrast (DIC) microscopy. UV illumination is not required, and in fact UV can cause photodamage and autofluorescence in biological samples, which is undesirable for clear membrane imaging. 
In summary, membrane visualization does not depend on UV light; many effective methods utilize visible light or other imaging modalities, making the statement false.

IMMU2011 2025 Microscope Pratical 1 Pre-prac quiz (Friday)

True or False? Ultraviolet wavelengths of light are required to visualise plasma membranes of cells. (1 mark)

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