In a flow-through chromatography step (e.g., AEX polishing in many mAb platforms), the intended operating mode is:

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BlackTom AI · Accepted Answer

The question concerns the intended operating mode in a flow-through chromatography step, such as AEX polishing used in monoclonal antibody platforms.

First, we note that in flow-through (also called pass-through) operation, the resin is chosen and conditioned so that the molecule of interest (e.g., the mAb) does not bind under the selected conditions, while undesired impurities have strong interactions with the resin and are retained. This enables the product to pass through in the effluent while impurities are captured on the resin.

In the context of AEX polishing, this typically means that the product flows through the column, and charged impurities (host cell proteins, DNA, aggregates, or other charged contaminants) are captured by the resin due to electrostatic interactions, whereas the product remains in the flow and exits the column.

Option analysis:
- If the option states that impurities bind to the resin while the product passes through in the effluent, this accurately describes the flow-through mode: the product is in the effluent and the impurities are retained on the resin. This aligns with the intended purpose of a polishing step designed to remove impurities without binding the product.

- If there were an alternate option suggesting that the product binds to the resin or that both product and impurities bind, or that the process relies on binding the product for purification, such statements would be inconsistent with the flow-through operating principle. In flow-through polishing, the product should not be captured by the resin under the chosen conditions; otherwise, yield would be compromised and the purification objective would not be met.

In summary, the core idea of flow-through purification is to keep the target molecule in the mobile phase while capturing impurities on the resin, thereby achieving impurity removal without product binding. This conceptual understanding supports the statement that impurities bind to the resin while the product passes through in the effluent.

2026WI_CHEM_ENG_377-0_SEC20_AND_CHEM_ENG_477-0_SEC20_AND_MBIOTECH_476-2_SEC1

In a flow-through chromatography step (e.g., AEX polishing in many mAb platforms), the intended operating mode is:

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