T/F: Whichever DNA strand is transcribed, the RNA polymerase reads the template strand from 3′ to 5′.

Question

BlackTom AI · Accepted Answer

Starting with the core concept of transcription directionality, RNA polymerase uses the DNA template strand to synthesize RNA in the 5' to 3' direction.

Option analysis, although there are no listed choices beyond True/False here: If we consider the statement that the RNA polymerase reads the template strand from 3' to 5', this aligns with the standard mechanism: the polymerase moves along the template in the 3' to 5' direction and creates a complementary RNA strand that runs 5' to 3'.

To clarify the related details, the template (antisense) strand is read 3'→5' by RNA polymerase. The resulting RNA is complementary and antiparallel, and thus its synthesis proceeds 5'→3'. The coding (sense) strand, which has the same sequence as the RNA (except T for U), is not the strand being read to guide synthesis.

If one were to entertain a false statement, such as reading the template in the opposite direction (5' to 3'), it would imply RNA synthesis in the wrong orientation or yield a nonfunctional transcript, which contradicts the fundamental mechanism of transcription.

Another potential misconception could be confusing directionality with where transcription starts or the orientation of the RNA relative to the DNA. In reality, directionality is fixed: read template 3'→5', synthesize RNA 5'→3'.

In summary, the statement reflects the accepted model of transcription: RNA polymerase reads the template strand from 3' to 5' and builds the RNA in the 5' to 3' direction.

251A-LIFESCI-7A-LEC-1 4.3 Transcription Review Questions

T/F: Whichever DNA strand is transcribed, the RNA polymerase reads the template strand from 3′ to 5′.

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