Both the alkaline lysis and zyppy DNA isolation protocols, use of isopropanol to achieve precipitation of DNA molecules.

Question

BlackTom AI · Accepted Answer

Examining the claim about DNA precipitation steps in two distinct protocols requires attention to the practical workflow differences between methods.
Option 1: 'True' — This would assert that both the alkaline lysis and Zyppy DNA isolation protocols rely on isopropanol to precipitate DNA. While some protocols do use isopropanol to precipitate nucleic acids, this statement generalizes to both methods. In reality, the Zyppy DNA isolation kit (from Zymo Research) typically uses a spin-column cleanup approach rather than a precipitation step with alcohol, meaning DNA binding and purification occur on a column rather than through isopropanol precipitation. Therefore, stating that both protocols use isopropanol precipitation is not universally accurate.
Option 2: 'False' — This option correctly signals that the broad claim is incorrect. The alkaline lysis method can involve alcohol precipitation (isopropanol or ethanol) in many traditional plasmid prep workflows, but the Zyppy workflow is designed to purify DNA via a column-based capture rather than requiring isopropanol precipitation. This distinction explains why the statement does not hold true across both protocols and highlights a key practical difference in purification strategy between the two methods.
In summary, the assertion that both protocols rely on isopropanol precipitation oversimplifies and misrepresents the Zyppy method, which commonly uses a column-based purification step instead of alcohol precipitation.

BCMB20005_2025_SM1 Lecture 2 Quiz

Both the alkaline lysis and zyppy DNA isolation protocols, use of isopropanol to achieve precipitation of DNA molecules.

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