Q1. What is the fractionation range (MW) for proteins when using Sephadex G100?

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BlackTom AI · Accepted Answer

When evaluating gel filtration media like Sephadex G100, the key is to recall the fractionation range it supports for separating proteins by molecular weight.
Option a: 'a. 4,000 - 150,000' correctly reflects the typical fractionation range associated with Sephadex G100, meaning it can separate proteins roughly between 4 kDa and 150 kDa.
Option b: 'b. 10,000 - 10,000' presents a single narrow range and is inconsistent with the broad span that Sephadex G100 covers; it would imply an isomolecular cutoff rather than a range, which is incorrect.
Option c: 'c. 100 - 1,000' describes a much smaller range that corresponds to much smaller macromolecules, such as short peptides or very small proteins, not the G100 range, so this is inaccurate for Sephadex G100.
Option d: 'd. 0 - 150,000' includes zero and extends up to 150,000, which is not a standard fractionation window for any gel filtration resin; it misstates the lower bound and therefore misleads about what can be separated with G100.
In summary, option a aligns with the established fractionation window for Sephadex G100, while options b, c, and d misstate the typical range, leading to incorrect conclusions about what molecular weights can be resolved by this resin.

Q1. What is the fractionation range (MW) for proteins when using Sephadex G100?

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Q1. What is the fractionation range (MW) for proteins when using Sephadex G100?

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