Q1. What is the fractionation range (MW) for proteins when using Sephadex G100?

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BlackTom AI · Accepted Answer

Let’s parse the question carefully and lay out what each option implies about the fractionation range (MW) for proteins when using Sephadex G100.
Option a: '10,000 - 10,000' suggests a single molecular weight value rather than a range, and an extremely narrow span that does not reflect typical gel filtration ranges. This is inconsistent with how Sephadex G100 separates proteins across a broad spectrum of sizes. The absence of a true range makes this option unreliable.
Option b: '0 - 150,000' would imply that proteins as small as zero molecular weight (essentially very tiny peptides) up to 150,000 Da could be resolved in one run. In reality, Sephadex G100 has a more limited upper and lower bound, and it does not accommodate zero-Da species; the lower limit is above very small peptides. This broad inclusive lower bound misrepresents what Sephadex G100 typically resolves.
Option c: '100 - 1,000' proposes a relatively narrow and low MW range, which would miss many small to mid-sized proteins and certainly not capture the majority of typical protein sizes encountered in many purification workflows. Such a narrow window does not align with the standard fractionation profile of Sephadex G100, which is designed for larger proteins as well.
Option d: '4,000 - 150,000' reflects a broad but realistic span for Sephadex G100, capturing small to mid-size to larger proteins. Gel filtration media like Sephadex G100 are characterized by their fractionation range, and for G100 this range is commonly cited around a few thousand to a couple hundred thousand daltons, aligning with 4,000–150,000 Da as a representative spectrum. This option fits the conventional understanding of the resin’s separation window and how proteins of various sizes would elute.
In summary, evaluating each choice against the typical fractionation behavior of Sephadex G100 shows that the ranges described in options a, b, and c fail to accurately reflect the practical MW range, whereas option d aligns with the standard expectations for this resin’s fractionation window.

BCH2011 S1 2025 Pre-lab 4: MCQ

Q1. What is the fractionation range (MW) for proteins when using Sephadex G100?

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