he gel filtration chromatogram shown above is from a mixture of proteins. Choose below the appropriate sizes for the peaks shown. The molecular mass is in kD.

Question

BlackTom AI · Accepted Answer

To tackle this question, I will first restate what the gel filtration chromatogram shows and how to interpret peak order.
- In gel filtration (size-exclusion), larger proteins elute earlier (smaller elution time) because they are excluded from the internal pores and pass through the column faster. Smaller proteins elute later (larger elution time).
- The chromatogram provided has five labeled peaks (1, 2, 3, 4, 5) with peak 1 occurring around 5 minutes (earliest among the labeled peaks) and peak 5 around 10–11 minutes (latest), with peaks 2–4 in between. Thus, peak 1 corresponds to the largest molecular mass among the detected species, and peak 5 corresponds to the smallest.

Option 1: '1. 70, 2. 30, 3. 50, 4. 75, 5.15'
- This option assigns peak 1 as 70 kD, peak 2 as 30 kD, peak 3 as 50 kD, peak 4 as 75 kD, and peak 5 as 15 kD.
- Problems: The order of masses across peaks 1–4 should generally show a decreasing mass with later peaks. Here, peak 4 is given a larger mass (75 kD) than peak 1 (70 kD) and peak 3 (50 kD), which contradicts the elution order observed (earlier peaks should be heavier, not heavier after later peaks). Therefore this option is inconsistent with the chromatogram’s elution sequence.

Option 2: '1. 70, 2. 30, 3. 50, 4. 75, 5.15' (identical to option 1)
- The same reasoning as option 1 applies. The elution order would imply peak 4 is heavier than peak 1, which does not align with the chromatogram where later peaks should generally be lighter.

Option 3: '1. 15, 2. 25, 3. 30, 4. 50, 5. 70'
- This assigns peak 1 as 15 kD and peak 5 as 70 kD. That would place the largest protein at the latest eluting peak, which contradicts size-exclusion principles (largest proteins elute first). Hence this option is incorrect.

Option 4: 'none of the answers make sense'
- Given that the first three options fail to match the correct elution order (largest to smallest across peaks 1 to 5), and the plausible correct ordering would have peak 1 as the largest mass and peak 5 as the smallest, option 4 remains the only viable choice if none of the provided sequences align with the expected elution pattern.

In summary, the chromatogram shows peak 1 as the earliest (largest mass), peaks 2–4 as progressively smaller species, and peak 5 as the smallest. Options 1–3 assign inconsistent masses that do not follow the expected order from largest to smallest across peaks, making them incorrect. Option 4 therefore aligns with the observation that none of the provided specific sequences correctly map to the elution order observed in the graph.

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he gel filtration chromatogram shown above is from a mixture of proteins. Choose below the appropriate sizes for the peaks shown. The molecular mass is in kD.

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