Question at position 88 Eukaryotic mRNAs that have not been spliced can be translated.TrueFalse

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Question: Eukaryotic mRNAs that have not been spliced can be translated. True/False

Option 1: True. This statement is misleading because in eukaryotic cells, pre-mRNA transcripts that have not undergone splicing are generally not exported from the nucleus to the cytoplasm where translation occurs. Splicing is part of the RNA processing steps that prepare mRNA for export and translation, including capping, polyadenylation, and removal of introns. Unspliced RNA often fails quality control checks and is degraded or retained in the nucleus, preventing translation in the cytoplasm.

Option 2: False. This option correctly reflects the reality that splicing is a key maturation step for most eukaryotic mRNAs prior to translation. Without splicing, the transcript is typically not competent for translation due to nuclear retention, surveillance mechanisms, and potential issues with 5' cap recognition and coding sequence integrity. Although there are a few unusual cases (e.g., certain intron-retaining transcripts that may be translated under specific conditions), the general rule in typical eukaryotic systems is that unspliced mRNA is not translated.

In sum, the claim in Option 1 conflicts with the standard understanding of eukaryotic mRNA processing and export, while Option 2 aligns with the conventional view that splicing is generally required for productive translation of eukaryotic mRNAs.

Question at position 88 Eukaryotic mRNAs that have not been spliced can be translated.TrueFalse

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