Question at position 10 When RNA splicing occurs:ribosomes are split in halfintrons are removed from mRNAit means that a poly-adenine tail has been added50s and 30s ribosomal subunits are joined togetherDNA ligase is required

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BlackTom AI · Accepted Answer

Question framing: This item asks about what RNA splicing entails and provides several statements to evaluate.
Option 1: 'ribosomes are split in half' -- This is inaccurate. Ribosomes do not get split during splicing; splicing is a nuclear RNA processing event, whereas ribosomes function in translation in the cytoplasm. The idea of splitting a ribosome is not part of the splicing process.
Option 2: 'introns are removed from mRNA' -- This is the core feature of RNA splicing: introns (noncoding regions) are excised from the pre-mRNA, and exons (coding regions) are joined to form the mature mRNA. This statement correctly captures the essence of splicing.
Option 3: 'it means that a poly-adenine tail has been added' -- The addition of a poly-A tail (polyadenylation) occurs after transcription and processing, but it is a distinct step from splicing. Splicing concerns removing introns and joining exons, not poly-A tail addition.
Option 4: '50s and 30s ribosomal subunits are joined together' -- In prokaryotes, 50S and 30S are ribosomal subunits, but their joining is part of ribosome assembly during translation initiation, not RNA splicing. Splicing occurs prior to export of mRNA to the cytoplasm for translation, and does not involve joining ribosomal subunits.
Option 5: 'DNA ligase is required' -- DNA ligase is an enzyme involved in DNA repair and replication, sealing nicks in the DNA backbone. It is not required for RNA splicing, which processes RNA rather than DNA.
Overall, each incorrect option either misattributes a different cellular process to RNA splicing or describes steps that occur outside the splicing mechanism. The correct understanding is that splicing removes introns from the pre-mRNA to produce mature mRNA, with exons joined together, enabling proper translation without the intronic sequences.

Question at position 10 When RNA splicing occurs:ribosomes are split in halfintrons are removed from mRNAit means that a poly-adenine tail has been added50s and 30s ribosomal subunits are joined togetherDNA ligase is required

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