The role of betamercaptoethanol and urea in Anfinsen's experiment is respectively

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Let's examine the question: The role of beta-mercaptoethanol and urea in Anfinsen's experiment is respectively

Option 1: 'denaturation of all types of bonds/no role' — This is incorrect. Beta-mercaptoethanol and urea do not denature all bonds, and they do have specific roles in the experiment. The statements are too general and ignore the distinct actions on covalent vs non-covalent interactions.

Option 2: 'denaturation of non-covalent bonds/denaturation of covalent bonds' — This is reversed. In Anfinsen’s context, urea disrupts non-covalent interactions such as hydrogen bonds, ionic interactions, and van der Waals contacts, while beta-mercaptoethanol reduces disulfide (covalent) bonds. So this exchange is not aligned with the actual roles.

Option 3: 'dentaturation of covalent bonds/denaturation of non-covalent bonds' — This option has a misspelling ('dentaturation' instead of 'denaturation'), but the intended content matches the scientific idea: beta-mercaptoethanol denatures (reduces) covalent disulfide bonds, and urea denatures non-covalent interactions. If we ignore the spelling error, the reasoning aligns with the known mechanism in which covalent bonds are targeted by beta-mercaptoethanol and non-covalent interactions by urea.

Option 4: 'denaturation of hydrogen bonds/denaturation of disulfide bonds' — This is partially plausible since urea does disrupt hydrogen bonds (a non-covalent interaction), but the second part claims denaturation of disulfide bonds, which beta-mercaptoethanol can affect by reducing them, so the pairing here implies both agents denature both types, which is inaccurate. Moreover, the question asks for the roles of each reagent in the specified order, and this option confuses which reagent acts on which bond type.

In summary, the most accurate pairing describes beta-mercaptoethanol targeting covalent disulfide bonds (via reduction) and urea disrupting non-covalent interactions (hydrogen bonds, ionic interactions, etc.). The correct idea is therefore captured by the option that states covalent bond denaturation by beta-mercaptoethanol and non-covalent bond denaturation by urea, despite a minor spelling issue in that option.

BIOL3611.13541.202610 ClassMeeting7

The role of betamercaptoethanol and urea in Anfinsen's experiment is respectively

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