Questions
Single choice
In SDS-PAGE, which protein migrate most quickly through the gel? Ones that are ______.
Options
A.smallest
B.have a lot of highly negative R-groups
C.have a lot of highly positive R-groups
D.have a lot of hydrophobic R-groups
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Step-by-Step Analysis
In SDS-PAGE, proteins are denatured and coated with the detergent SDS, which imparts a uniform negative charge proportional to their length. This means migration through the polyacrylamide gel primarily reflects size (length of the polypeptide chain) rat......Login to view full explanationLog in for full answers
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