SDS-PAGE is ____________ and separates molecules based on ____________

Question

SDS-PAGE is  ____________ and separates molecules based on ____________

BlackTom AI · Accepted Answer

To begin, consider what SDS-PAGE does and how it operates. 
Option 1 states: Denaturing; molecular weight. This is accurate because SDS-PAGE uses the anionic detergent SDS to denature proteins and impart a uniform negative charge, allowing separation primarily by size (molecular weight) as they migrate through the gel. 
Option 2 claims: Non-denaturing; hydrophobicity and molecular weight. This is incorrect because SDS-PAGE is explicitly denaturing, not preserving native structure, and the separation is not based on hydrophobicity; it's driven mainly by size. 
Option 3 asserts: Non-denaturing; molecular weight. While molecular weight matters in this context, the method is not non-denaturing; the essential feature is the denaturing treatment with SDS, so this option misstates both the denaturation status and the basis of separation. 
Option 4 proposes: Denaturing; charge, hydrophobicity and molecular weight. Although it correctly notes denaturation, it overemphasizes multiple factors. In SDS-PAGE, the detergent SDS largely masks intrinsic charge and hydrophobicity, yielding separation mainly by molecular weight, not by a combination of charge or hydrophobicity. 
Taken together, the combination in Option 1 aligns with the standard description of SDS-PAGE’s denaturing protocol and size-based separation.

CHEM108_WI25_A00 Final Exam

SDS-PAGE is and separates molecules based on

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