Questions
BIOL3611.13541.202610 FA2IndFA2025- Requires Respondus LockDown Browser
Single choice
the SDS-PAGE gel shown above is the result of an experiment in which a ~55kDa protein is purified from cells, shown in Lane 3. What would you predict lanes 4, 5, and 6 show about the ~55kDa protein? Choose the correct answer from the answers below
Options
A.Lane 4, 5, and 6 are unrelated to lane 3
B.The 55kDa protein is made up of smaller subunits shown in lane 4, and independently in lanes 5 and 6
C.Proteins in Lane 4 are phosphorylated while the ones in the other lanes are not
D.There is no possible relationship between the proteins shown
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Step-by-Step Analysis
Begin by parsing the scenario: an SDS-PAGE gel is used to analyze a purified ~55 kDa protein from cells, shown in lane 3. The question asks what lanes 4, 5, and 6 would show about the same protein.
Option 1: 'Lane 4, 5, and 6 are unrelated to lane 3.' In SDS-PAGE, if lane 3 contains the purified protein, related lanes (4–6) typically examine the same protein under different conditions or forms. Saying they are unrelated ignores the experimental design and the expected relationship between......Login to view full explanationLog in for full answers
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