Watch the following pipetting aspiration video (1min 4sec):

video
Links to an external site.

Select the correct actions to achieve accurate pipetting (there is more than one, and both must be selected to achieve full marks. An incorrect selection will result in a deduction of marks):

Question

Watch the following pipetting aspiration video (1min 4sec):

video
Links to an external site.

Select the correct actions to achieve accurate pipetting (there is more than one, and both must be selected to achieve full marks. An incorrect selection will result in a deduction of marks):

BlackTom AI · Accepted Answer

Let's begin by restating what the question is asking and what information is available.
The prompt describes a pipetting aspiration video and asks you to select the correct actions to achieve accurate pipetting. It also notes there can be more than one correct action.
However, the provided data includes an empty set of answer options, which means we don't have the actual list of possible actions to evaluate. Despite this, we can still discuss the two actions listed in the "answer" field as what a student might consider selecting, and explain why they are or are not typically considered correct in the context of accurate pipetting.

Option 1: "Pipette with smooth action"
This suggestion aligns with standard best practices for accurate pipetting. Using a smooth, controlled motion helps minimize velocity fluctuations that can cause inconsistent aspiration and dispensing. Jerky or abrupt movements can introduce air bubbles, inaccurate volumes, and variability between trials. Therefore, describing the action as smooth is generally correct and supports measurement accuracy.

Option 2: "Maintain speed when pressing plunger"
The intent behind this option could be to promote a consistent rate of plunger depression. In many pipetting guidelines, a controlled and steady depress-and-release sequence is recommended to avoid creating turbulence or sudden pressure changes that could affect the volume drawn or expelled. However, the exact meaning of "maintain speed" matters:
- If interpreted as pressing the plunger smoothly and at a constant, moderate speed, this can be compatible with accurate pipetting.
- If interpreted as forcing a constant, perhaps overly rapid depression regardless of liquid type or pipette calibration, that could reduce accuracy and increase variability.
In typical practice, the goal is a smooth, controlled press and release, not a rapid or abrupt one. So the concept behind maintaining a controlled speed can be correct, but the phrasing may be ambiguous and could be misinterpreted as implying a particular speed rather than a focus on smoothness and control.

Without the actual list of answer options, we cannot definitively label which of the listed choices are correct for this specific question. The core ideas to evaluate in any correct answer would be (a) ensuring the pipette operates with smooth, controlled motions, and (b) executing the plunger depressions and releases in a steady, deliberate manner to avoid introducing errors. Any option asserting jerky, inconsistent, or uncontrolled motions would typically be incorrect, while options endorsing smooth operations and controlled plunger movement would be correct.

If you obtain the actual answer choices, you can apply these criteria: 
- Prefer options that emphasize smooth, continuous handling of the pipette and avoidance of abrupt or variable motions.
- Favor descriptions that specify controlled, consistent plunger depression and release, rather than fast or irregular actions.
- Be cautious of options that imply speed should be kept constant in a way that could encourage haste or error, unless they clearly tie that speed to a controlled, deliberate technique.

BCMB20005_2025_SM1 Week-1 Pre-Lab Quiz

Watch the following pipetting aspiration video (1min 4sec): video Links to an external site. Select the correct actions to achieve accurate pipetting (there is more than one, and both must be selected to achieve full marks. An incorrect selection will result in a deduction of marks):

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