Applying paper chromatography to separate the four substances below indicate where each substance will end up, starting from the bottom of the paper. (MW = Molecular Weight)
a. Midazolam, MW= 525.77

b. Niridazole, MW= 214.20

c. Propiomazine, MW= 440.40

d. Streptomycin, MW= 681.57

Question

Applying paper chromatography to separate the four substances below indicate where each substance will end up, starting from the bottom of the paper. (MW = Molecular Weight)
a. Midazolam, MW= 525.77

b. Niridazole, MW= 214.20

c. Propiomazine, MW= 440.40

d. Streptomycin, MW= 681.57

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Question restated: Applying paper chromatography to separate the four substances, indicate where each substance will end up starting from the bottom of the paper. Substances: a) Midazolam (MW = 525.77), b) Niridazole (MW = 214.20), c) Propiomazine (MW = 440.40), d) Streptomycin (MW = 681.57). Answer choices: 1) d, c, b, a 2) a, b, c, d 3) d, a, c, b

Option-by-option analysis:
- Option 1: d, c, b, a. This would place Streptomycin at the bottom, followed by Propiomazine, then Niridazole, with Midazolam at the top. If we assume that mobility on a paper chromatography plate correlates with a combination of polarity and interaction with the stationary phase, this sequence would suggest Streptomycin migrates least, and Midazolam migrates most. Given Streptomycin is highly polar and large, it commonly interacts strongly with the silica (stationary phase) and tends to stay near the origin (bottom). However, placing Niridazole ahead of Propiomazine (b before c) would imply Niridazole travels less than Propiomazine despite Niridazole’s relatively small size; without full polarity data, this order may conflict with typical trends where smaller, less polar molecules often travel farther. Overall, this arrangement seems inconsistent with a straightforward low-to-high mobility pattern for a mixture containing these compounds.

- Option 2: a, b, c, d. This order would place Midazolam at the bottom and Streptomycin at the top, implying the heaviest, most polar compound (Streptomycin) moves farthest. This contradicts the common expectation that highly polar, bulky molecules interact strongly with the stationary phase and stay closer to the origin, not farthest up the plate. Therefore this sequence is unlikely given the chemistry involved.

- Option 3: d, a, c, b. This sequence sets Streptomycin at the bottom (least mobile), Midazolam next, Propiomazine next, and Niridazole at the top (most mobile). This aligns with a plausible interpretation: Streptomycin’s high polarity and bulky structure give it strong retention on silica, keeping it near the origin. Midazolam, being relatively lipophilic, would migrate farther than Streptomycin but less than a smaller or differently polar compound. Propiomazine would be intermediate, and Niridazole, being smaller and comparatively less retained in some solvent systems, could migrate the farthest. While the exact order can depend on the solvent system and the exact polarities and functional groups, this linear progression from least to most mobile matches a reasonable expectation for these compounds on many silica-based plates.

Reasoning about the incorrect options in light of general chromatography principles:
- Option 1 (d, c, b, a) places Niridazole ahead of Propiomazine and Midazolam ahead of Streptomycin in a way that may not reflect typical retention given Streptomycin’s strong interactions with silica and the relative polarities. It also disrupts a smooth gradient of mobility that would typically accompany increasing mobility from a polar, bulky molecule to smaller or less polar ones.
- Option 2 (a, b, c, d) reverses the expected direction for the most mobile versus least mobile given the polarities and molecular weights; assigning the heaviest, most polar Streptomycin to the top contradicts the common behavior of silica chromatography where such a compound stays near the origin.

Putting it together: among the provided choices, Option 3 (d, a, c, b) offers a sequence that more consistently reflects a plausible bottom-to-top progression based on the general interplay of polarity, molecular size, and stationary-phase interactions on a typical silica plate. The order starts with Streptomycin at the bottom due to strong silica interaction, followed by Midazolam with greater mobility, then Propiomazine, and ends with Niridazole at the top as the most mobile in this arrangement.

BIOL-10-20048-S25 Lab Quiz 1 - Monday 3/3

Applying paper chromatography to separate the four substances below indicate where each substance will end up, starting from the bottom of the paper. (MW = Molecular Weight) a. Midazolam, MW= 525.77 b. Niridazole, MW= 214.20 c. Propiomazine, MW= 440.40 d. Streptomycin, MW= 681.57

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