In order to be able to visualize the stain under a microscope, you need to use....

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The question asks: 'In order to be able to visualize the stain under a microscope, you need to use....' This type of prompt typically tests understanding of which microscope objective and technique are required to clearly view stained specimens.

First, consider what is generally required to visualize a stain well. Stains are often visualized with higher magnification objectives that provide enough resolving power and numerical aperture to detect contrast between stained structures and surrounding material. In many light microscopy contexts, the highest practical single objective used for viewing stained specimens is the 100x objective, frequently used with immersion oil to improve light collection and resolution. This means the key requirement is selecting the appropriate high-magnification objective (commonly 100x) and applying immersion oil when applicable, rather than alternating between two different objectives in a single step.

Now evaluate the provided option: '40x lens then the 100x lense.' This phrasing suggests switching lenses sequentially during viewing. In standard practice, you choose one objective at a time and focus progressively, rather than passing through multiple objectives to see the same field. While you might start with a 40x objective to locate the region of interest and then switch to 100x for final focusing and visualization, the instruction implies that the essential requirement to visualize the stain is the use of the high-magnification objective (and oil immersion if applicable), not a workflow that combines them in a single visualization step. Misconception here is thinking both lenses must be used in tandem for visualization rather than recognizing the purpose of the higher magnification step.

If we consider typical alternative answers (which are not shown here), plausible correct statements would emphasize:
- Using the 100x objective with immersion oil to maximize resolution for stained specimens.
- Ensuring proper illumination and contrast (e.g., brightfield or phase-contrast as appropriate) to make the stain visible.
- Beginning with a lower magnification to locate the sample, then switching to high magnification for detail (without implying that both must be used at once).

The absence of visible answer options here makes it impossible to definitively label which choice is correct. However, from a correctness standpoint, the central idea is that visualization of a stain typically requires a high-magnification objective (often 100x) and correct immersion conditions if an oil-immersion objective is used, rather than a rigid claim that you must always use 40x followed by 100x in sequence as a fixed protocol.

In summary, if an option states 'use the 100x oil-immersion objective (with proper illumination) to visualize the stain,' that would align with standard microscopy practice. An option suggesting that two objectives must be used in a fixed order for visualization would be less precise, though it could describe a common workflow for locating a field before high-magnification examination. Without the actual answer choices, the critical concept to take away is the role of the high-magnification objective (often 100x with immersion oil) in visualizing stained specimens, complemented by appropriate illumination and focusing steps.

BIOL-1407-078 Bacterial Staining Follow Up

In order to be able to visualize the stain under a microscope, you need to use....

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