What are TRUE about the resolution of a light microscope? Select all the apply.

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Question restatement: What statements are TRUE about the resolution of a light microscope? Select all that apply.

Options:
- It is related to the numerical aperture of the eyepiece.
- It can be improved by moving to longer wavelengths.
- It is the ability to differentiate two distinct points very close to each other.
- It is limited by light diffraction.

Analysis of each option:
- Option: It is related to the numerical aperture of the eyepiece.
  This is not correct. Resolution in light microscopy is primarily determined by the numerical aperture of the objective lens (which collects light) and the wavelength of light used. The eyepiece contributes largely to magnification and viewing comfort, not to the fundamental resolution limit. Misconception here conflates eyepiece properties with optical resolving power.

- Option: It can be improved by moving to longer wavelengths.
  This is false. According to the diffraction limit, resolution improves with shorter wavelengths of light. Using longer wavelengths would increase the minimum resolvable distance, making it worse, not better. The inverse relationship between wavelength and resolution is well established in optical theory.

- Option: It is the ability to differentiate two distinct points very close to each other.
  This statement is true. A key definition of resolution in microscopy is the smallest distance at which two points can be distinctly perceived as separate. This directly reflects the resolving power of the instrument.

- Option: It is limited by light diffraction.
  This statement is true. The diffraction of light sets the fundamental limit to resolution in a conventional light microscope, described by criteria such as the Abbe limit, which ties resolution to wavelength and numerical aperture. Diffraction is the core physical constraint.

Putting it together, the true statements are those that identify resolution as (a) the ability to distinguish two close points, and (b) as limited by diffraction. The other two options misattribute factors or suggest incorrect wavelength dependence. The explanations above align with standard optical principle about resolution limits and what components influence them.

BN5102 Quiz 3

What are TRUE about the resolution of a light microscope? Select all the apply.

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