A study by Peluso et al. 2013 investigated the relationship between temperature and aggression in Caribbean flamingos. During each observation period, the researchers recorded the temperature, the number of flamingos, and each aggressive behavior 'instance' observed. Figure 1 below shows the relationship between temperature and the number of aggressive behaviors.

Citation: Peluso et al. 2013. Avian Biology Research 6:215-220.

Based on the data presented, what type of analysis is most appropriate to evaluate their research question?

Question

A study by Peluso et al. 2013 investigated the relationship between temperature and aggression in Caribbean flamingos. During each observation period, the researchers recorded the temperature, the number of flamingos, and each aggressive behavior 'instance' observed. Figure 1 below shows the relationship between temperature and the number of aggressive behaviors.

Citation: Peluso et al. 2013. Avian Biology Research 6:215-220.

Based on the data presented, what type of analysis is most appropriate to evaluate their research question?

BlackTom AI · Accepted Answer

To approach the question, we start by identifying the variables involved: temperature is a continuous predictor (in Celsius) and the outcome is the number of aggressive instances, a count that varies across observation periods. 
Option 1: Linear regression. This analysis is appropriate when you want to assess whether a continuous independent variable (temperature) is associated with a continuous or count outcome (number of aggressive instances). Even though counts can be modeled with Poisson or negative binomial regression, a simple linear regression can be used to evaluate a linear relationship if the data meet the assumptions (linearity, homoscedasticity, etc.). The figure showing a potential linear trend between temperature and aggression supports considering a regression approach to quantify the slope and strength of the association. 
Option 2: T test. A t-test compares the means between two groups. Here there aren’t two clearly defined groups based on a categorical variable; instead, the research question involves how a continuous variable (temperature) relates to another variable (aggressive instances). Thus, a t-test is not appropriate for testing the temperature–aggression relationship in this context. 
Option 3: Chi-square. The chi-square test is used for associations between categorical variables or for goodness-of-fit tests. Both temperature and aggression counts are not categorical variables in this setup (temperature is continuous, aggression is a count), so a chi-square test would not correctly address the research question. 
In summary, given the study design aims to examine how a continuous predictor (temperature) relates to a numerical outcome (number of aggressive instances), linear regression is the most fitting initial analytical choice, with caveats about counts and potential alternative models if overdispersion or nonlinearity is present.

BIOLOGY 1114.01 SP2025 (17927) 1114 Comprehensive Lab Final Exam - Requires Respondus LockDown Browser

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