When using the UV lamp and viewing your Thin Layer Chromatography (TLC) results, what safety precautions should you observe?

Question

BlackTom AI · Accepted Answer

Question restatement: When using the UV lamp and viewing your Thin Layer Chromatography (TLC) results, what safety precautions should you observe? Answer options:
- a. Wear gloves when handling the TLC plate.
- b. Use the UV lamp in a darkened room.
- c. Do not expose eyes and skin to the UV light.
- d. Both a. and c.

Option-by-option analysis:
Option a: 'Wear gloves when handling the TLC plate.' Wearing gloves helps protect your hands from potential chemical residues on the TLC plate or solvents used in TLC development. This is a sensible safety practice and is commonly recommended in laboratory protocols to prevent skin contact with hazardous substances. It is a valid precaution.

Option b: 'Use the UV lamp in a darkened room.' Using a UV lamp in a darkened room can help with visibility of the TLC plate under UV light, but the statement speaks to the environment rather than a direct safety precaution. The focus of safety guidelines is typically protection from UV exposure, handling of reagents, and eye/skin shielding rather than merely reducing ambient light. Therefore, while this may be a practical viewing condition, it is not primarily a safety precaution per se.

Option c: 'Do not expose eyes and skin to the UV light.' UV exposure can damage eyes and skin; this is a clear safety precaution. Protecting eyes and skin from UV radiation is a fundamental safety consideration when working with UV lamps.

Option d: 'Both a. and c.' This option asserts that the appropriate precautions include wearing gloves during handling and protecting eyes/skin from UV exposure. Since both of these are valid safety measures, this combined option accurately represents the safety expectations described in standard lab practice regarding TLC under UV light.

Summary of reasoning across options: A is correct as a gloves-on handling precaution. B discusses the viewing environment rather than a safety precaution, making it less directly relevant to safety. C is correct as it addresses direct protection from UV exposure. D combines the two valid safety precautions (A and C) into one option, aligning with standard safety recommendations for TLC under UV light.

MCD4400 - T3 - 2025 Prelab Quiz - Esterification - Benzocaine

When using the UV lamp and viewing your Thin Layer Chromatography (TLC) results, what safety precautions should you observe?

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