The pI is the pH where a molecules charge is overall neutral . This is sometimes at near pH 7.

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The question describes the isoelectric point (pI) as the pH at which a molecule carries no net charge. This is the defining idea for pI, so the statement that the pI corresponds to a neutral overall charge is correct.

Option: neutral
This option directly states the core definition of pI: at the pH where the molecule has zero net charge, the overall charge is neutral. This aligns with established concepts in biochemistry and protein chemistry, where the pI marks the point of no net charge and minimal overall ionic migration in an electric field.

Option: sometimes
The second statement adds a nuance: for some molecules, or under certain conditions, the pI can be near pH 7. This is often true for many amino acids and proteins that have balanced acidic and basic groups, resulting in a pI around neutral pH. However, the exact pI varies widely across different molecules, and for others it can be much lower or higher than 7 depending on their side chains and overall composition.

In summary, recognizing that the pI corresponds to neutral charge is essential, and acknowledging that the pI can often be near neutral pH for some molecules captures the variability observed in real-world cases. The combination of these two statements reflects both the fundamental definition and its contextual variability across different molecules.

CHE 008B A01 SS1 2025 Lecture 15 -pI and Isoelectric Point

The pI is the pH where a molecules charge is overall neutral . This is sometimes at near pH 7.

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