Which of the following is least likely to be involved in whole-genome sequencing?

Question

BlackTom AI · Accepted Answer

The question asks which option is least likely to be involved in whole-genome sequencing, so we need to evaluate each proposed method.
Option 1: 'map-based sequencing' involves constructing a genome sequence using a genetic map to order and orient sequence data; this method is a recognized approach in some historical and contemporary genome projects, so it is plausible in the context of whole-genome sequencing.
Option 2: 'All of these approaches are common in whole-genome sequencing.' This statement would be true only if every listed method is indeed commonly used; since one of the options is not a standard sequencing approach, this blanket claim becomes dubious.
Option 3: 'shotgun sequencing' is a widely used, foundational approach for assembling entire genomes by randomly breaking DNA into small fragments and then reassembling the sequences computationally; it is clearly a standard technique in WGS.
Option 4: 'Maxim-Gilbert sequencing' appears to be a fictitious or non-existent sequencing method. It does not correspond to any established or widely recognized sequencing technology, standards, or protocols in the field, making it the least likely to be involved in whole-genome sequencing.
In summary, the first two options reflect concepts that can be associated with genome sequencing in some contexts, and shotgun sequencing is a canonical WGS method, whereas Maxim-Gilbert sequencing lacks basis in current genomics methods; therefore, it is the least likely option among those listed.

BIOL_2004_81774_202509 Homework 4

Which of the following is least likely to be involved in whole-genome sequencing?

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