In genome construction, what are contigs assembled into?

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BlackTom AI · Accepted Answer

In genome assembly discussions, various terms describe different levels of assembled sequences.
Option 1: Libraries. While libraries are the prepared DNA fragments used to generate sequencing reads, they are not the assembled units that contigs are grouped into. This choice confuses the input material with the assembly product.
Option 2: Gene predictions. Gene prediction is an annotation step that follows assembly, where potential genes are identified within the assembled sequences. It is not the structural unit that contigs form during assembly.
Option 3: Paired-end reads. Paired-end reads provide linkage information to help assembly, but they themselves are not structures that contigs are assembled into. They are the data used to build contigs, not the final higher-level assembly product.
Option 4: Scaffolds. This is the correct conceptual step: contigs are ordered and oriented relative to each other and linked into scaffolds, often with gaps representing unknown sequences. Scaffolds unify contigs into larger, span-containing constructs.
Option 5: Transcripts. Transcripts arise from expressed RNA and are the product of transcriptional activity, not a structural result of contig assembly in genome projects.

AGRI20044_2025_SM1 Sample Final Exam Questions

In genome construction, what are contigs assembled into?

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