In the field of genomics, what is Genome Assembly?

Question

In the field of genomics, what is Genome Assembly?

BlackTom AI · Accepted Answer

The question asks for the definition of Genome Assembly in genomics, so we need to evaluate each option against the standard meaning in the field.
Option 1: 'The laboratory method where an organism's genome is prepared to be read by a sequencing instrument.' This describes sample preparation or library preparation steps, not assembly itself. It’s about making DNA ready for sequencing rather than assembling reads into a genome.
Option 2: 'The process of creating multiple copies of a genome.' This corresponds more to amplification or cloning concepts (like PCR amplification) and does not capture the assembly task of reconstructing the genome from fragments.
Option 3: 'The process of predicting the biological function of genes within a genome.' This aligns with genome annotation or functional genomics, not assembly. It involves assigning roles to genes, not assembling sequence data.
Option 4: 'The process of aligning and merging overlapping DNA fragments that are part of a genome.' This precisely matches the definition of genome assembly: taking short DNA reads or fragments and computationally aligning and merging them to reconstruct the original genome sequence.
In summary, the first three options describe preparation, amplification, or annotation aspects of genomics, which are distinct from assembly. The fourth option accurately captures the core concept of genome assembly: stitching together overlapping fragments to form the complete genomic sequence.

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In the field of genomics, what is Genome Assembly?

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