What mapping technique is not a type of physical mapping in eukaryotes?

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BlackTom AI · Accepted Answer

The question asks which mapping technique is not a type of physical mapping in eukaryotes, so we need to evaluate each option in terms of whether it measures physical distances on chromosomes or relies on other principles.
Option 1: somatic cell hybridization. This method involves fusing cells and tracking the presence or absence of genes across hybrids to assign genes to chromosomes; it directly informs physical localization on chromosomes and is considered a form of physical mapping.
Option 2: deletion mapping. By analyzing which genes are lost together when specific chromosomal regions are deleted, researchers can infer the physical order and location of genes along a chromosome. This is a classic physical mapping approach.
Option 3: recombinational frequency mapping. This technique uses the frequency of recombination between markers during meiosis to estimate genetic distances (centimorgans). It reflects genetic linkage rather than a direct physical distance on the chromosome, so it is not a physical mapping method.
Option 4: in situ hybridization. This technique uses labeled DNA or RNA probes to locate specific sequences on chromosomes within cells, providing a direct physical location, hence a physical mapping method.
Thus, while all other methods provide physical localization or distance information on the chromosome, recombinational frequency mapping relies on recombination data to infer genetic distance, not a direct physical map.

BIOL_2004_81774_202509 Homework 2

What mapping technique is not a type of physical mapping in eukaryotes?

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