In a flow-through chromatography step (e.g., AEX polishing in many mAb platforms), the intended operating mode is:

Question

BlackTom AI · Accepted Answer

The question concerns the intended operating mode in a flow-through chromatography step, such as AEX polishing used in monoclonal antibody platforms.

First, we note that in flow-through (also called pass-through) operation, the resin is chosen and conditioned so that the molecule of interest (e.g., the mAb) does not bind under the selected conditions, while undesired impurities have strong interactions with the resin and are retained. This enables the product to pass through in the effluent while impurities are captured on the resin.

In the context of AEX polishing, this typically means that the product flows through the column, and charged impurities (host cell proteins, DNA, aggregates, or other charged contaminants) are captured by the resin due to electrostatic interactions, whereas the product remains in the flow and exits the column.

Option analysis:
- If the option states that impurities bind to the resin while the product passes through in the effluent, this accurately describes the flow-through mode: the product is in the effluent and the impurities are retained on the resin. This aligns with the intended purpose of a polishing step designed to remove impurities without binding the product.

- If there were an alternate option suggesting that the product binds to the resin or that both product and impurities bind, or that the process relies on binding the product for purification, such statements would be inconsistent with the flow-through operating principle. In flow-through polishing, the product should not be captured by the resin under the chosen conditions; otherwise, yield would be compromised and the purification objective would not be met.

In summary, the core idea of flow-through purification is to keep the target molecule in the mobile phase while capturing impurities on the resin, thereby achieving impurity removal without product binding. This conceptual understanding supports the statement that impurities bind to the resin while the product passes through in the effluent.

2026WI_CHEM_ENG_377-0_SEC20_AND_CHEM_ENG_477-0_SEC20_AND_MBIOTECH_476-2_SEC1

In a flow-through chromatography step (e.g., AEX polishing in many mAb platforms), the intended operating mode is:

View Explanation

Log in for full answers

Similar Questions

In a consumer society, many adults channel creativity into buying things

Economic stress and unpredictable times have resulted in a booming industry for self-help products

People born without creativity never can develop it

A product has a selling price of $20, a contribution margin ratio of 40% and fixed cost of $120,000. To make a profit of $30,000. The number of units that must be sold is: Type the number without $ and a comma. Eg: 20000

Which of the following statement regarding cost is correct:

Under the assumptions used in cost-volume-profit analysis, as the activity increases:

The CVP and break-even analysis are supported by a range of important assumptions. Which of the following is not an assumption of the CVP:

The break-even point is that at which:

More Practical Tools for Students Powered by AI Study Helper

Homework AI Solver

Stylized AI Paper Writer

Plagiarism Checker Assistant

Citation AI Academic Writing Tool

In-Class Translation Assistant

AI Note Generator

AI Quiz Answers

Past Exam Questions from University Test Bank

Smart Practice Assistant

Adaptive Practice

Making Your Study Simpler