What is the mRNA complement to the DNA template 5’-CGCATGTAGCGA-3’?

Question

What is the mRNA complement to the DNA template 5’-CGCATGTAGCGA-3’?

BlackTom AI · Accepted Answer

To determine the mRNA complement to the given DNA template, we must apply base pairing rules and consider the directionality of transcription.
Option 1: 5’-GCGTACATCGCT-3’. This answer uses thymine (T) in the RNA sequence, but RNA contains uracil (U) instead of thymine. Additionally, it presents a 5’ to 3’ orientation that does not reflect the complementary, antiparallel relationship to the DNA template as required. Therefore this is incorrect.
Option 2: 3’-AGCGATGTACGC-5’. Here, there is a mismatch in base pairing for several positions (e.g., A pairing with U in RNA, not with T or C as shown). It also begins with A rather than providing the correct complementary bases to the template sequence when read in the proper orientation. These issues render it incorrect.
Option 3: 5’-AGCGATGTACGC-3’. This option correctly shows an RNA sequence, but its orientation and nucleotide order do not align with the actual complementary RNA produced from the given template. It would also require replacing T with U in RNA and reordering to reflect the antiparallel transcription process. Hence, this is incorrect.
Option 4: 3’-GCGUACAUCGCU-5’. This matches the proper Watson–Crick base pairing for RNA (G with C, C with G, A with U, T with A in RNA, etc.) and reflects the antiparallel orientation to the DNA template (the RNA is written 3’ to 5’ to align with the template’s 5’ to 3’ direction during transcription). It also correctly uses uracil (U) instead of thymine (T). Therefore this is the correct complement.
In summary, the RNA complement must be written in a way that is antiparallel to the DNA template and use U in place of T, which is consistently satisfied by option 4 and not by the others.

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What is the mRNA complement to the DNA template 5’-CGCATGTAGCGA-3’?

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