A section of a template strand of a gene is shown below.

3`–T-A-C-G-C-T-A-A-C–5`

Which of the following represents the correct mRNA sequence transcribed from this segment?

Question

A section of a template strand of a gene is shown below.

3`–T-A-C-G-C-T-A-A-C–5`

Which of the following represents the correct mRNA sequence transcribed from this segment?

BlackTom AI · Accepted Answer

To determine the mRNA sequence, recall that transcription uses the DNA template strand to build an RNA strand in the 5' to 3' direction, with RNA bases pairing as follows: A pairs with U, T pairs with A, C pairs with G, and G pairs with C. The given template strand runs 3'–T A C G C T A A C–5'. If we write the complementary RNA sequence in the 5' to 3' direction, we replace T with A, A with U, C with G, and G with C accordingly, yielding 5'–A U G C G A U U G–3'. Now let's evaluate the options.

Option 1: 5–A–U–G–C–G–A–U–U–G–3. This sequence exactly matches the expected RNA complement in the correct 5' to 3' orientation derived from the template. It uses A, U, G, and C in the precise order that follows the base-pairing rules with the given template.

Option 2: 5–G–U–U–A–G–C–G–C–A–3. Here the first base is G instead of A, which already indicates a mismatch at the first position relative to the template, and the rest of the sequence does not align with the derived complement, so this option fails the base-pairing consistency.

Option 3: 5–T–A–C–G–C–T–A–A–C–3. This sequence contains thymine (T) instead of uracil (U) and also mirrors the DNA template rather than representing RNA, so it cannot be the mRNA transcript.

Option 4: 5–A–T–G–C–G–A–T–T–G–3. This option has thymine (T) in multiple positions where uracil (U) should appear in an RNA transcript, and the overall order does not match the transcriptional complement of the provided template strand.

In summary, the only sequence that faithfully represents the mRNA transcript produced from this template, respecting base-pairing rules and the 5' to 3' direction, is the one that shows A at the first position followed by U, G, C, G, A, U, U, G in that exact order. The other options deviate by using T instead of U where appropriate, by starting with a different base, or by presenting a sequence that mirrors the DNA template rather than the RNA product.

Bio 93 Section CDE 2025 Midterm 2- Section E

A section of a template strand of a gene is shown below. 3`–T-A-C-G-C-T-A-A-C–5` Which of the following represents the correct mRNA sequence transcribed from this segment?

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