If a segment of DNA is 5'-TAC GAT TAG-3', the RNA that results from the transcription of this segment will be

Question

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We start by restating the problem and all answer options clearly.
Question: If a segment of DNA is 5'-TAC GAT TAG-3', the RNA that results from the transcription of this segment will be
Options: 
1) 3'-TAC GAT TAU-5' 
2) 3'-AAC GAU UAA-5' 
3) 3'-UAC GAU UAG-5' 
4) 3'-AUG CUA AUC-5' 
5) 3'-ATG CTA ATA-5'

Now, evaluate each option step by step.
Option 1: 3'-TAC GAT TAU-5'. This choice uses thymine (T) in the RNA sequence, but RNA contains uracil (U) instead of thymine. That makes this option biologically incorrect for RNA. Also the sequence does not match the complementary pairing rules for transcription from the given DNA segment.
Option 2: 3'-AAC GAU UAA-5'. Here, some bases align with potential complementarity, but the overall sequence does not correspond to the correct transcription product from either the template or coding strand when using standard base-pairing and orientation conventions. The presence of UAA (a stop-like sequence in many contexts) is not derived directly from the provided DNA segment in a consistent transcriptional read.
Option 3: 3'-UAC GAU UAG-5'. This sequence is the RNA complement written in the 3' to 5' orientation of the transcription product. If you take the given DNA 5'-TAC GAT TAG-3' and determine its transcription product on the opposite (template) strand, you obtain a transcript that is complementary in RNA terms and typically presented 5'→3'. Writing it 3'→5' yields 3'-UAC GAU UAG-5', which matches the expected complement but reversed orientation.
Option 4: 3'-AUG CUA AUC-5'. This option is the RNA sequence that is complementary to the provided DNA segment when you consider the template strand and express the transcript in the conventional orientation (3'→5' for the template, which corresponds to 5'→3' for the RNA). Since transcription uses the template strand in 3'→5' direction, the RNA product is complementary and would be written 5'→3' as AUG CUA AUC; presenting it as 3'-AUG CUA AUC-5' reflects the same sequence in the opposite orientation. In many question formats, this orientation is accepted as the correct transcription product, and this option is presented as the correct choice.
Option 5: 3'-ATG CTA ATA-5'. This sequence contains thymine bases (T) and does not correspond to an RNA transcript (no U in place of T), making it invalid as an RNA product from transcription.

In summary, each incorrect option either misrepresents RNA (using T instead of U), misorients the transcript (wrong 3' vs 5' direction), or mismatches base-pairing logic. The correct transcription product, when oriented consistently with how transcription products are usually presented (and as given in the provided answer set), is 3'-AUG CUA AUC-5', which corresponds to the complementary RNA sequence to the given DNA segment when viewed from the appropriate strand orientation.

APBIO142-030 with labs S17 - CollegeBoard* AP Exam Prep: Multiple Choice

If a segment of DNA is 5'-TAC GAT TAG-3', the RNA that results from the transcription of this segment will be

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