After decanting the liquid into the column, the DNA is bound with the first spin (after the neutralization step). What is used as the washing solution?

Question

BlackTom AI · Accepted Answer

In this question, we’re asked to identify the washing solvent used after binding DNA to the column during a spin-column purification workflow.
Option 1: high salt solution. This is the correct choice because, after binding DNA in a high-salt binding buffer, the column is typically washed with a high-salt wash to remove impurities while keeping DNA bound to the silica matrix. The high ionic strength helps disrupt non-specifically bound contaminants.
Option 2: 5% ethanol. Ethanol is sometimes used in some protocols for precipitation steps or specific wash steps in alternative purification methods, but in standard silica-based spin-column DNA purifications, ethanol is not used as the routine wash performing the same function as the high-salt wash.
Option 3: DNA staining dyes. DNA staining dyes are used for visualization or quantification after extraction (e.g., in gels or fluorescent assays), not as washing buffers within the column purification workflow. Using a dye as a wash would contaminate the sample and defeat the purpose of purification.
Option 4: SDS. SDS is a detergent used to lyse cells or denature proteins; it is not used as a washing solution in the standard DNA column purification workflow. Including SDS during washing would likely disrupt DNA binding and contaminate the product.
Overall, the high salt wash is the standard step to remove impurities while maintaining DNA binding, making it the correct option among the listed choices.

After decanting the liquid into the column, the DNA is bound with the first spin (after the neutralization step). What is used as the washing solution?

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