Question at position 46 Consider this single stranded DNA sequence: 3’ A G C A T C 5’Which of the following would be the complementary strand? 5' C T A C A G 3'5' G A U G C T 3'5' U C G U A G 3'5' T C G T A G 3'5' G A C A T C 3'

Question

Question at position 46 Consider this single stranded DNA sequence: 3’ A  G  C  A  T  C  5’Which of the following would be the complementary strand? 5' C  T  A  C  A  G 3'5' G  A  U  G  C  T 3'5' U  C  G  U  A  G 3'5' T  C  G  T  A  G 3'5' G  A  C  A  T  C 3'

BlackTom AI · Accepted Answer

Let's break down the problem by identifying base-pairing rules and applying them to the given sequence.
First, note the given strand is 3' A G C A T C 5'. In DNA, A pairs with T and G pairs with C. The complementary strand should be written 5' to 3' so we arrange the partner bases in the order that pairs with the template.
Now evaluate each option:
- Option 1: 5' C T A C A G 3'. Here, the sequence begins with C pairing to G on the template, which is inconsistent with the required A paired with T and G paired with C rule. The overall order does not align with the complementary logic for the entire strand.
- Option 2: 5' G A U G C T 3'. This option contains a U (uracil), which only appears in RNA. Since the question concerns DNA strands, including U makes this option incorrect on the basis of molecular type.
- Option 3: 5' U C G U A G 3'. This also uses U, making it an RNA-type sequence, not DNA. Additionally, the arrangement does not correspond to the correct DNA complement for the given template.
- Option 4: 5' T C G T A G 3'. This matches the expected complementary bases in order: A pairs with T, G with C, C with G, A with T, T with A, C with G, yielding exactly 5' T C G T A G 3'. This aligns with the 3' to 5' template when read in the 5' to 3' direction of the new strand.
- Option 5: 5' G A C A T C 3'. This is simply the reverse of part of the original sequence and does not reflect the proper complementary pairing to form a stable DNA duplex with the given template. It also leaves mismatches for several bases.
In summary, the only option that correctly applies A↔T and G↔C pairings while presenting the strand in the correct 5'→3' orientation is Option 4. The other choices either introduce RNA bases, misorder the pairings, or fail to correspond to the complementary relationship dictated by the template strand.

Question at position 46 Consider this single stranded DNA sequence: 3’ A G C A T C 5’Which of the following would be the complementary strand? 5' C T A C A G 3'5' G A U G C T 3'5' U C G U A G 3'5' T C G T A G 3'5' G A C A T C 3'

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