Using the DNA strand shown below, what is the sequence of the complementary strand?

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Start by noting that DNA base pairing is specific: A pairs with T, and G pairs with C. 
Next, identify the orientation of the given strand in the problem. If the prompt provides a sequence on one strand and asks for the complementary strand, you must construct the partner strand by substituting each base with its pair and then flipping the direction to produce the complementary sequence in the opposite 5' to 3' orientation. 
In this case, the provided information indicates the complementary strand sequence is 5'–AGCCAT–3'. To verify conceptually, work letter by letter: A pairs with T, so wherever you see T on the original strand, the complementary would have an A; G pairs with C, so a G on the original corresponds to a C on the complement, and so on. If the original strand runs in the opposite orientation, the resulting complementary strand must be written 5' to 3' accordingly. 
Consider common pitfalls: - Forgetting the 5' to 3' direction when writing the complementary strand. - Mispairing A with C or G with T. - Writing the complement in the same orientation as the original instead of in the opposite orientation. 
Additionally, some problems provide multiple-choice options; when options are present, compare each candidate to the correct base-pairing rules and the required 5' to 3' orientation to determine which one matches. 
If no options are shown, you can still apply the rule directly: substitute each base with its pair and ensure the final strand is written in the correct 5' to 3' direction, reflecting the complementary nature of the two strands. 
In summary, constructing a complementary strand involves strict base-pairing rules and a careful attention to strand orientation, ensuring that A pairs with T and G pairs with C, and that the final sequence is presented in the opposite orientation to the original strand.

BIOL_1105_60452_202506 Lesson 11 Homework

Using the DNA strand shown below, what is the sequence of the complementary strand?

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