If we added 15 µL of 60 ng/µL pGLO solution to our transformation reaction, how many µg of DNA did we add?

Question

BlackTom AI · Accepted Answer

Let’s break down the data we’re given and perform the unit math carefully.
First, identify the DNA concentration and the volume added: 60 ng/µL and 15 µL.
Next, multiply concentration by volume to get total DNA mass: 60 ng/µL × 15 µL = 900 ng.
Convert 900 ng to micrograms since the answer choices are in µg: 900 ng = 0.900 µg, i.e., 0.9 µg.
Now evaluate each option:
- Option '9': This would imply 9 µg of DNA. To check, 9 µg would correspond to 9,000 ng; dividing by 15 µL would require a concentration of 600 ng/µL, which is not the given 60 ng/µL. So this is incorrect.
- Option '900': Interpreting as 900 µg would be orders of magnitude larger than the supplied material (900 ng total). Since 60 ng/µL × 15 µL = 900 ng, 900 µg is not possible here. Incorrect.
- Option '4': This would be 4 µg, equivalent to 4,000 ng. Again, this does not match the calculated 900 ng; incorrect.
- Option '0.9': This exactly matches the computed total mass in micrograms (0.9 µg). The math 60 ng/µL × 15 µL = 900 ng = 0.9 µg supports this value. This option is correct.
- Option '0.25': This would be 0.25 µg, or 250 ng. That would require a product of concentration and volume equal to 250 ng, which is not our given values. Incorrect.
- Option '90': Interpreting as 90 µg would require 90,000 ng total, far beyond 900 ng calculated from the given numbers. Incorrect.
In summary, only the calculation 60 ng/µL × 15 µL yields 900 ng, which is 0.9 µg. The remaining options are inconsistent with the provided concentration and volume, either by overestimating or underestimating the total DNA mass.

W25 BIOL D006B 01Y Cell & Molecular Biology Lab Quiz 4

If we added 15 µL of 60 ng/µL pGLO solution to our transformation reaction, how many µg of DNA did we add?

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