Advantages of using the biotin-avidin system include: (choose all that apply)

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Question restatement: The topic asks for advantages of using the biotin-avidin system and specifies to choose all that apply. The two candidates provided are:
- Rapid and irreversible bond formation
- Amplification of signal

Option 1 – Rapid and irreversible bond formation: The biotin-avidin interaction is renowned for its exceptionally high affinity, making the binding extremely strong and effectively permanent under many laboratory conditions. This high affinity enables rapid complex formation once biotin is presented to avidin. However, while the bond is often described as effectively irreversible under typical assay conditions, it is technically a very strong non-covalent interaction rather than a true chemical covalent bond. The practical takeaway is that the binding occurs quickly and remains stable, which is a major advantage in assay reliability and sensitivity.

Option 2 – Amplification of signal: The biotin-avidin system is widely exploited to amplify signals in various detection methods. Because avidin (or streptavidin) has multiple biotin-binding sites, it can be used to attach multiple reporter molecules (enzymes, fluorophores, or labels) to a single biotinylated molecule, thereby increasing signal strength. This multivalency is a core reason why the system enhances detection sensitivity and is considered a major advantage in assays like ELISA, blotting, and immunohistochemistry.

In summary, Option 1 correctly highlights the strong, rapid binding characteristic of biotin-avidin, contributing to stability of the complex under assay conditions. Option 2 correctly highlights the signal amplification capability due to multivalency and the ability to recruit multiple labeling entities. A common misconception to avoid is overcalling the bond 'irreversible' as truly permanent; it is best described as extremely stable under typical experimental conditions, which is sufficient for most practical purposes.

BIOT5750.39480.202530 Module 4 Quiz S25

Advantages of using the biotin-avidin system include: (choose all that apply)

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