Below are illustrated five plates prepared using the same protocol used in the pGLO experiment.

(Note that the plate numbers here do not correspond to the plate numbers in your lab manual tables.)

In which plate do we expect to see no bacterial colonies?

Question

Below are illustrated five plates prepared using the same protocol used in the pGLO experiment.

(Note that the plate numbers here do not correspond to the plate numbers in your lab manual tables.)

In which plate do we expect to see no bacterial colonies?

BlackTom AI · Accepted Answer

To tackle this question, I’ll go through each plate option and reason about what each label implies for bacterial growth under the given conditions.
Option plate 1: labeled 'pGLO+ Ampicillin No arabinose'. Since these cells carry the pGLO plasmid and are grown on ampicillin-containing medium, they should be able to survive the antibiotic. However, because arabinose is absent, GFP expression would not be induced, so colonies would grow but without GFP fluorescence. This plate is expected to show colonies.
Option plate 2: labeled 'no pGLO + Ampicillin'. Here there is no pGLO plasmid, but ampicillin is present. Without the plasmid, the bacteria cannot express AmpR to resist ampicillin, so colonies would not be expected to form. This plate is unlikely to show colonies due to lack of antibiotic resistance genes.
Option plate 3: labeled 'pGLO+ Ampicillin Arabinose'. This plate has the plasmid and antibiotic, and includes arabinose to induce GFP expression. Therefore, colonies should grow and fluoresce under appropriate conditions. This plate is expected to show colonies (with GFP).
Option plate 4: labeled 'pGLO− Ampicillin No arabinose'. This plate lacks the pGLO plasmid entirely, yet still contains ampicillin. Without the plasmid-encoded AmpR gene, these cells cannot resist the antibiotic, so no colonies should form. This plate is the one without colonies.
Option plate 5: labeled 'no pGLO Ampicillin No arabinose'. Similar to plate 2, there is no pGLO plasmid, and ampicillin is present. Without AmpR, these cells cannot grow, so this plate would also be expected to have no colonies; however, the explicit comparison indicates plate 4 is the plate where growth is absent due to the plasmid absence combined with antibiotic pressure.
In summary, plate 4 is the plate where we expect no bacterial colonies to appear because the bacteria lack the pGLO plasmid (and thus the Ampicillin resistance gene) while ampicillin is present, preventing growth. The other plates either contain the plasmid (with or without induction) or lack the antibiotic pressure in a way that would permit growth based on the given labels.

W25 BIOL D006B 01Y Cell & Molecular Biology Lab Quiz 3

Below are illustrated five plates prepared using the same protocol used in the pGLO experiment. (Note that the plate numbers here do not correspond to the plate numbers in your lab manual tables.) In which plate do we expect to see no bacterial colonies?

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