In this mixed culture, which pairing of A and B is MOST consistent with both morphology and Gram reaction?

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When evaluating the pairing of A and B in this mixed culture, the first step is to connect each organism to its typical morphology and Gram reaction.
Option 1 claims A = intracellular cocci and B = extracellular rods. This is inconsistent with standard bacterial shapes and placements: Staphylococcus are not intracellular cocci, and while Bacillus are rods, the clue of being extracellular is not the defining feature used to differentiate these two genera in mixed cultures.
Option 2 swaps the identities: A = Bacillus (rods) and B = Staphylococcus (cocci). While this matches their general shapes, the option asserts B as Staphylococcus and A as Bacillus but does not align with the common phrasing used for a mixed-culture reasoning that matches the organism arrangements typically seen in diagnostic images. It also ignores endospore-forming traits that Bacillus can exhibit.
Option 3 states A = Bacillus and B = Staphylococcus, with A described as rods and B as cocci, but then claims B is Gram-negative cocci. That is incorrect because Staphylococcus species are Gram-positive cocci, never Gram-negative, so this option contains a factual mismatch about Gram reaction.
Option 4 presents A = Staphylococcus and B = Bacillus, supported by the reasoning that A are clusters of Gram-positive cocci and B are Gram-positive rods that may show endospores. This aligns with canonical morphology: Staphylococcus forms grape-like clusters of Gram-positive cocci, while Bacillus appears as Gram-positive rods and many species form endospores, which is a key distinguishing feature.

In summary, the correct pairing should reflect both morphology and Gram staining: Staphylococcus as Gram-positive cocci in clusters, and Bacillus as Gram-positive rods with potential endospores. The fourth option matches these characteristics, whereas the other options contain contradictions in shape, Gram reaction, or key identifying traits.

MIMI2002 (ND) Microbiology Practical Quiz

In this mixed culture, which pairing of A and B is MOST consistent with both morphology and Gram reaction?

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